Beschreibung:
AbstractDetails for predicting secondary structure of RNA sequences using free energy minimization are given. Protocols present the use of the computer program RNAstructure (for PCs) and the mfold server (for Unix platforms). The minimum free energy structure and a set of suboptimal structures with similar free energies are predicted. In addition, a triangular plot called the energy dot plot is predicted. This shows the superposition of all possible base pairs within a prescribed free energy increment.