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Kambouris, Nicholas G.; Burke, Daniel J.; Creutz, Carl E.

Cloning and genetic characterization of a calcium‐ and phospholipid‐binding protein from Saccharomyces cerevisiae that is homologous to translation elongation factor‐1γ

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  • Medientyp: E-Artikel
  • Titel: Cloning and genetic characterization of a calcium‐ and phospholipid‐binding protein from Saccharomyces cerevisiae that is homologous to translation elongation factor‐1γ
  • Beteiligte: Kambouris, Nicholas G.; Burke, Daniel J.; Creutz, Carl E.
  • Erschienen: Wiley, 1993
  • Erschienen in: Yeast
  • Sprache: Englisch
  • DOI: 10.1002/yea.320090206
  • ISSN: 0749-503X; 1097-0061
  • Schlagwörter: Genetics ; Applied Microbiology and Biotechnology ; Biochemistry ; Bioengineering ; Biotechnology
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  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>We have isolated a gene (<jats:italic>CAM1</jats:italic>) from the yeast <jats:italic>Saccharomyces cerevisiae</jats:italic> that encodes a protein homologous to the translational cofactor elongation factor‐1γ (EF‐1γ) first identified in the brine shrimp <jats:italic>Artemia salina</jats:italic>. The predicted Cam1 amino acid sequence consists of 415 residues that share 32% identity with the <jats:italic>Artemia</jats:italic> protein, increasing to 72% when conservative substitutions are included. The calculated M<jats:sub>r</jats:sub> of Cam1p (47 092 Da) is in close agreement with that of EF‐1γ (M<jats:sub>r</jats:sub> = 49 200 Da), and hydropathy plots of each protein exhibit strikingly similar profiles. Disruption of the <jats:italic>CAM1</jats:italic> locus yields four viable meiotic progeny, indicating that under normal growth conditions the Cam1 protein is non‐essential. Attempts to elicit a translational phenotype have been unsuccessful. Since EF‐1γ participates in the regulation of a GTP‐binding protein (EF‐1α), double mutants with <jats:italic>cam1</jats:italic> disruptions and various mutant alleles of known GTP‐binding proteins were constructed and examined. No evidence was found for an interaction of <jats:italic>CAM1</jats:italic> with <jats:italic>TEF1, TEF2, SEC4, YPT1, RAS1, RAS2, CDC6, ARF1, ARF2</jats:italic> or <jats:italic>CIN4</jats:italic>. The possibility that Cam1p may play a redundant role in the regulation of protein synthesis or another GTP‐dependent process is discussed.</jats:p>