> Detailanzeige

Sreeramulu, Sridhar; Richter, Christian; Berg, Hannes; Wirtz Martin, Maria A.; Ceylan, Betül; Matzel, Tobias; Adam, Jennifer; Altincekic, Nadide; Azzaoui, Kamal; Bains, Jasleen Kaur; Blommers, Marcel J. J.; Ferner, Jan; Fürtig, Boris; Göbel, Michael; Grün, J. Tassilo; Hengesbach, Martin; Hohmann, Katharina F.; Hymon, Daniel; Knezic, Bozana; Martins, Jason N.; Mertinkus, Klara R.; Niesteruk, Anna; Peter, Stephen A.; Pyper, Dennis J.; [...]

Exploring the Druggability of Conserved RNA Regulatory Elements in the SARS‐CoV‐2 Genome

Literatur-
verwaltung

Zur
Merkliste

Lösche von
Merkliste

Per Whatsapp teilen

Schließen

Merkliste



Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
  • Medientyp: E-Artikel
  • Titel: Exploring the Druggability of Conserved RNA Regulatory Elements in the SARS‐CoV‐2 Genome
  • Beteiligte: Sreeramulu, Sridhar; Richter, Christian; Berg, Hannes; Wirtz Martin, Maria A.; Ceylan, Betül; Matzel, Tobias; Adam, Jennifer; Altincekic, Nadide; Azzaoui, Kamal; Bains, Jasleen Kaur; Blommers, Marcel J. J.; Ferner, Jan; Fürtig, Boris; Göbel, Michael; Grün, J. Tassilo; Hengesbach, Martin; Hohmann, Katharina F.; Hymon, Daniel; Knezic, Bozana; Martins, Jason N.; Mertinkus, Klara R.; Niesteruk, Anna; Peter, Stephen A.; Pyper, Dennis J.; [...]
  • Erschienen: Wiley, 2021
  • Erschienen in: Angewandte Chemie International Edition
  • Sprache: Englisch
  • DOI: 10.1002/anie.202103693
  • ISSN: 1521-3773; 1433-7851
  • Schlagwörter: General Chemistry ; Catalysis
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>SARS‐CoV‐2 contains a positive single‐stranded RNA genome of approximately 30 000 nucleotides. Within this genome, 15 RNA elements were identified as conserved between SARS‐CoV and SARS‐CoV‐2. By nuclear magnetic resonance (NMR) spectroscopy, we previously determined that these elements fold independently, in line with data from in vivo and <jats:italic>ex‐vivo</jats:italic> structural probing experiments. These elements contain non‐base‐paired regions that potentially harbor ligand‐binding pockets. Here, we performed an NMR‐based screening of a poised fragment library of 768 compounds for binding to these RNAs, employing three different <jats:sup>1</jats:sup>H‐based 1D NMR binding assays. The screening identified common as well as RNA‐element specific hits. The results allow selection of the most promising of the 15 RNA elements as putative drug targets. Based on the identified hits, we derive key functional units and groups in ligands for effective targeting of the RNA of SARS‐CoV‐2.</jats:p>