Beschreibung:
<jats:sec><jats:title>Premise of The Study</jats:title><jats:p>Simple sequence repeat (<jats:styled-content style="fixed-case">SSR</jats:styled-content>) markers were developed for the study of genetic diversity of New Zealand <jats:italic>Nassella trichotoma</jats:italic> (Poaceae) and to support future studies in its native range.</jats:p></jats:sec><jats:sec><jats:title>Methods and Results</jats:title><jats:p>Genomic <jats:styled-content style="fixed-case">DNA</jats:styled-content> was extracted from <jats:italic>N. trichotoma</jats:italic> leaf material and subjected to Roche 454 sequencing. From a total of 745 putative <jats:styled-content style="fixed-case">SSR</jats:styled-content>s, 48 with di‐ to pentanucleotide repeats were screened, 32 primer pairs were designed, and 15 polymorphic markers were optimized for multiplex <jats:styled-content style="fixed-case">PCR</jats:styled-content> on 105 <jats:italic>N. trichotoma</jats:italic> samples from four New Zealand regions. Each locus resulted in two to six alleles per locus, and four of the loci cross‐amplified in <jats:italic>N. tenuissima</jats:italic>. The mean observed and expected heterozygosity ranged from 0.00 to 0.90 and 0.00 to 0.50 per locus, respectively.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The novel <jats:styled-content style="fixed-case">SSR</jats:styled-content> markers are valuable for the study of genetic diversity of <jats:italic>N. trichotoma</jats:italic> and might also be useful for closely related species.</jats:p></jats:sec>