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Medientyp: E-Artikel Titel: Ethanol production from paper sludge by simultaneous saccharification and co‐fermentation using recombinant xylose‐fermenting microorganisms Beteiligte: Zhang, Jiayi; Lynd, Lee R. Erschienen: Wiley, 2010 Erschienen in: Biotechnology and Bioengineering Sprache: Englisch DOI: 10.1002/bit.22811 ISSN: 0006-3592; 1097-0290 Schlagwörter: Applied Microbiology and Biotechnology ; Bioengineering ; Biotechnology Entstehung: Anmerkungen: Beschreibung: <jats:title>Abstract</jats:title><jats:p>Simultaneous saccharification and co‐fermentation (SSCF) of waste paper sludge to ethanol was investigated using two recombinant xylose‐fermenting microbes: <jats:italic>Zymomonas mobilis</jats:italic> 8b and <jats:italic>Saccharomyces cerevisiae</jats:italic> RWB222. <jats:italic>S. cerevisiae</jats:italic> RWB222 produced over 40 g/L ethanol with a yield of 0.39 g ethanol/g carbohydrate on paper sludge at 37°C, while similar titers and yields were achieved by <jats:italic>Z. mobilis</jats:italic> 8b at 30°C. Both <jats:italic>S. cerevisiae</jats:italic> RWB222 and <jats:italic>Z. mobilis</jats:italic> 8b exhibited decreasing cell viability at 37°C when producing over 40 g/L ethanol. A high ethanol concentration can account for <jats:italic>S. cerevisiae</jats:italic> RWB222 viability loss, but ethanol concentration was not the only factor influencing <jats:italic>Z. mobilis</jats:italic> 8b viability loss at 37°C. Over 3 g/L residual glucose was observed at the end of paper sludge SSCF by <jats:italic>Z. mobilis</jats:italic> 8b, and a statistical analysis revealed that a high calcium concentration originating from paper sludge, a high ethanol concentration, and a high temperature were the key interactive factors resulting in glucose accumulation. The highest ethanol yields were achieved by SSCF of paper sludge with <jats:italic>S. cerevisiae</jats:italic> RWB222 at 37°C and <jats:italic>Z. mobilis</jats:italic> 8b at 30°C. With good sugar consumption at 37°C, <jats:italic>S. cerevisiae</jats:italic> RWB222 was able to gain an improvement in the polysaccharide to sugar yield compared to that at 30°C, whereas <jats:italic>Z. mobilis</jats:italic> 8b at 30°C had a lower polysaccharide to sugar yield, but a higher sugar to ethanol yield than <jats:italic>S. cerevisiae</jats:italic>. Both organisms under optimal conditions achieved a 19% higher overall conversion of paper sludge to ethanol than the non‐xylose utilizing <jats:italic>S. cerevisiae</jats:italic> D5A at its optimal process temperature of 37°C. Biotechnol. Bioeng. 2010;107: 235–244. © 2010 Wiley Periodicals, Inc.</jats:p>