Small extracellular vesicles derived from human adipose‐derived stem cells regulate energetic metabolism through the activation of YAP/TAZ pathway facilitating angiogenesis
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Medientyp:
E-Artikel
Titel:
Small extracellular vesicles derived from human adipose‐derived stem cells regulate energetic metabolism through the activation of YAP/TAZ pathway facilitating angiogenesis
Beschreibung:
<jats:title>Abstract</jats:title><jats:p>Recent studies have found small extracellular vesicles (sEVs) that are secreted from human adipose tissue‐derived stem cells (hADSCs‐sEVs) and contribute to angiogenesis. Glycolysis, the primary energetic pathway of vascular endothelial cells, plays a key role in the process of angiogenesis. However, hADSCs‐sEVs' effects on energy metabolism within endothelial cells remain unclear. In our study, we found that hADSCs‐sEVs restored glycolytic metabolism suppressed by 3‐(pyridinyl)‐1‐(4‐pyridinyl)‐2‐propen‐1‐one(3PO), a unique glycolytic inhibitor increasing the extracellular acidification rate (ECAR), oxygen consumption rate (OCR), glycolytic gene expression as well as pyruvate, lactate, and ATP production in HUVEC cells. In contrast, hADSCs‐sEVs decreased PDH‐E1α expression and acetyl‐CoA production. The above results indicate that hADSCs‐sEVs promote HUVEC angiogenesis via enhancing glycolysis and suppressing mitochondrial oxidative phosphorylation. Furthermore, we found that the YAP/TAZ pathway may play a key role in the effects hADSCs‐sEVs have on HUVECs, thus, providing a promising approach for pro‐angiogenesis‐related regeneration.</jats:p>