Düppe, Peter M.;
Talbierski, Peter M.;
Hornig, Frank S.;
Rauen, Ursula;
Korth, Hans‐Gert;
Wille, Timo;
Boese, Roland;
Omlor, Thorsten;
de Groot, Herbert;
Sustmann, Reiner
Pyrene‐Based Fluorescent Nitric Oxide Cheletropic Traps (FNOCTs) for the Detection of Nitric Oxide in Cell Cultures and Tissues
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Medientyp:
E-Artikel
Titel:
Pyrene‐Based Fluorescent Nitric Oxide Cheletropic Traps (FNOCTs) for the Detection of Nitric Oxide in Cell Cultures and Tissues
Beteiligte:
Düppe, Peter M.;
Talbierski, Peter M.;
Hornig, Frank S.;
Rauen, Ursula;
Korth, Hans‐Gert;
Wille, Timo;
Boese, Roland;
Omlor, Thorsten;
de Groot, Herbert;
Sustmann, Reiner
Erschienen:
Wiley, 2010
Erschienen in:
Chemistry – A European Journal, 16 (2010) 36, Seite 11121-11132
Beschreibung:
AbstractThe synthesis and the structural and spectroscopic characterization of nonfluorescent, pyrene‐based cyclico‐quinodimethanes are reported. These compounds react efficiently with nitric oxide (NO) in a formal cheletropic manner, by which the fluorescent aromatic pyrene system is regenerated. The NO trapping capabilities and kinetics of the fluorescent nitric oxide cheletropic traps (FNOCTs) are assessed in THF and buffered aqueous solution by ESR, UV/Vis, and fluorescence spectroscopy, by employing NO solutions and NO released fromN‐diazeniumdiolates (NONOates). Prototypal biological applications include the quantitation of NO production from cultured rat alveolar macrophages and the endothelium of porcine aorta, which demonstrate a sensitivity for NO detection in the nanomolar range.