Guanosine in a Single Stranded Region of Anticodon Stem–Loop tRNA Models is Prone to Oxidatively Generated Damage Resulting in Dehydroguanidinohydantoin and Spiroiminodihydantoin Lesions
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E-Artikel
Titel:
Guanosine in a Single Stranded Region of Anticodon Stem–Loop tRNA Models is Prone to Oxidatively Generated Damage Resulting in Dehydroguanidinohydantoin and Spiroiminodihydantoin Lesions
Beschreibung:
<jats:title>Abstract</jats:title><jats:p>Oxidation of RNA hairpin models corresponding to anticodon stem–loop (ASL) of transfer RNA led to RNA damage consisting solely of a unique loop guanine oxidation. Manganese porphyrin/oxone treatment of RNA resulted in dehydroguanidinohydantoin (<jats:bold>DGh</jats:bold>; major) and/or spiroiminodihydantoin (<jats:bold>Sp</jats:bold>) lesions. Ribose damage was not observed. This two‐electron transfer oxidation reaction allowed the identification of guanine oxidation products for further study of RNA species carrying a unique lesion at a single G to investigate their biological impact.</jats:p>