The human kinesin‐14 HSET tracks the tips of growing microtubules in vitro

Medientyp: E-Artikel
Titel: The human kinesin‐14 HSET tracks the tips of growing microtubules in vitro
Beteiligte: Braun, Marcus; Lansky, Zdenek; Bajer, Seweryn; Fink, Gero; Kasprzak, Andrzej A.; Diez, Stefan
Erschienen: Wiley, 2013
Erschienen in: Cytoskeleton, 70 (2013) 9, Seite 515-521
Sprache: Englisch
DOI: 10.1002/cm.21133
ISSN: 1949-3584; 1949-3592
Schlagwörter: Cell Biology ; Structural Biology
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Beschreibung: <jats:p>Tip‐tracking of kinesin‐14 motor proteins is believed to be crucial for the assembly and maintenance of dynamic microtubule arrays. However, in contrast to other members of the kinesin‐14 family, <jats:italic>H. sapiens</jats:italic> kinesin‐14 HSET has so far never been observed to be prominently located at microtubule plus ends. Here, using an in vitro microtubule dynamics reconstitution assay we observe tip‐tracking of GFP‐HSET in the presence of <jats:italic>H. sapiens</jats:italic> EB1 (hsEB1). Tip‐tracking depended on the SxIP‐like motif in HSET as well as on the EB homology domain in hsEB1. <jats:italic>D. melanogaster</jats:italic> Ncd and <jats:italic>S. pombe</jats:italic> Klp2 tip‐tracking reconstitution assays accompanied by kinesin‐14 amino acid sequence comparisons suggest that SxIP‐like motif mediated tip‐tracking dependent on EB family proteins is conserved in the kinesin‐14 family of molecular motors. © 2013 Wiley Periodicals, Inc.</jats:p>

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