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Medientyp: E-Artikel Titel: A highly sensitive particle agglutination assay for the detection of P53 autoantibodies in patients with lung cancer Beteiligte: Agaylan, Ashraf; Binder, Daniel; Sauer, Markus; Neuweiler, Hannes; Meyer, Oliver; Kiesewetter, Holger; Salama, Abdulgabar Erschienen: Wiley, 2007 Erschienen in: Cancer Sprache: Englisch DOI: 10.1002/cncr.23057 ISSN: 0008-543X; 1097-0142 Schlagwörter: Cancer Research ; Oncology Entstehung: Anmerkungen: Beschreibung: <jats:title>Abstract</jats:title><jats:sec><jats:title>BACKGROUND.</jats:title><jats:p>Numerous assays have been described for the detection of p53 autoantibodies. These assays are highly specific with low sensitivity. In this report, the authors describe a highly sensitive and simple particle agglutination immunoassay using superparamagnetic particles for capturing p5 autoantibodies, p53 protein, and p53 protein‐antibody complexes from large volumes of serum samples (2 mL).</jats:p></jats:sec><jats:sec><jats:title>METHODS.</jats:title><jats:p>Superparamagnetic particles were coated with different peptides spanning the entire p53 protein. These particles were incubated with serum samples from healthy blood donors (n = 180), from patients without malignancies (n = 27), and from patients with various forms of lung cancer (n = 166). The particles were washed and placed into the reaction chamber of a gel card. After centrifugation, agglutination results were read visually. Positive reactions were defined by a layer of particles on top of the gel or agglutinated particles dispersed through the gel matrix.</jats:p></jats:sec><jats:sec><jats:title>RESULTS.</jats:title><jats:p>Depending on the peptide used, p53 autoantibodies were detected in from 17.5% to 35% of the investigated patients with lung cancer. By using a commercially available enzyme‐linked immunoadsorbent assay (ELISA) kit, p53 autoantibodies were detected in only 3% of those patients. P53 protein and p53 protein‐antibody complexes were not detected in patients with lung cancer (n = 20).</jats:p></jats:sec><jats:sec><jats:title>CONCLUSIONS.</jats:title><jats:p>The newly developed assay was easy to perform and had sensitivity superior to that of the currently available p53 ELISAs. Cancer 2007. © 2007 American Cancer Society.</jats:p></jats:sec> Zugangsstatus: Freier Zugang