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Medientyp: E-Artikel Titel: Comprehensive Protocols for CRISPR/Cas9‐based Gene Editing in Human Pluripotent Stem Cells Beteiligte: Santos, David P.; Kiskinis, Evangelos; Eggan, Kevin; Merkle, Florian T. Erschienen: Wiley, 2016 Erschienen in: Current Protocols in Stem Cell Biology Sprache: Englisch DOI: 10.1002/cpsc.15 ISSN: 1941-7322; 1938-8969 Entstehung: Anmerkungen: Beschreibung: <jats:title>Abstract</jats:title><jats:p>Genome editing of human pluripotent stem cells (hPSCs) with the CRISPR/Cas9 system has the potential to revolutionize hPSC‐based disease modeling, drug screening, and transplantation therapy. Here, we aim to provide a single resource to enable groups, even those with limited experience with hPSC culture or the CRISPR/Cas9 system, to successfully perform genome editing. The methods are presented in detail and are supported by a theoretical framework to allow for the incorporation of inevitable improvements in the rapidly evolving gene‐editing field. We describe protocols to generate hPSC lines with gene‐specific knock‐outs, small targeted mutations, or knock‐in reporters. © 2016 by John Wiley & Sons, Inc.</jats:p>