Beschreibung:
<jats:p>Phase diagrams including the metastable zone width for the L‐asparaginase II from <jats:italic>Escherichia coli</jats:italic> with both PEG<jats:sub>6000</jats:sub> and ethanol as a precipitant agent were determined, respectively. A turbidity technique giving results of nucleation and solubility limits in hours rather than in days was introduced. For the first time such data necessary for designing and controlling of crystallization processes of L‐asparaginase II are presented. Since the catalytical activity is an important parameter for L‐asparaginase II as an enzyme, a new type of phase diagram plotting the enzymatic activity as a function of temperature was established as well. Additionally, the crystalline L‐asparaginase II crystals with different shapes that formed in PEG<jats:sub>6000</jats:sub> and ethanol, respectively, were also investigated by X‐ray Powder Diffraction (XRPD).</jats:p>