> Detailanzeige

Hartung, Susann; Rauh, Christopher; Hoang, Thi Ngoc Mai; Jahreis, Susanne; Wagner, Kathleen; Macheleidt, Juliane; Brakhage, Axel A.; Rummler, Silke; Hochhaus, Andreas; von Lilienfeld‐Toal, Marie

Fast and Quantitative Evaluation of Human Leukocyte Interaction with Aspergillus fumigatus Conidia by Flow Cytometry

Literatur-
verwaltung

Zur
Merkliste

Lösche von
Merkliste

Per Whatsapp teilen

Schließen

Merkliste



Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
  • Medientyp: E-Artikel
  • Titel: Fast and Quantitative Evaluation of Human Leukocyte Interaction with Aspergillus fumigatus Conidia by Flow Cytometry
  • Beteiligte: Hartung, Susann; Rauh, Christopher; Hoang, Thi Ngoc Mai; Jahreis, Susanne; Wagner, Kathleen; Macheleidt, Juliane; Brakhage, Axel A.; Rummler, Silke; Hochhaus, Andreas; von Lilienfeld‐Toal, Marie
  • Erschienen: Wiley, 2019
  • Erschienen in: Cytometry Part A
  • Sprache: Englisch
  • DOI: 10.1002/cyto.a.23653
  • ISSN: 1552-4922; 1552-4930
  • Schlagwörter: Cell Biology ; Histology ; Pathology and Forensic Medicine
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>Systemic infections with the opportunistic mold <jats:italic>Aspergillus fumigatus</jats:italic> are a great threat to immunocompromised patients such as transplant recipients. Immunological research on <jats:styled-content style="fixed-case"><jats:italic>A. fumigatus</jats:italic></jats:styled-content> involves the measurement of phagocytosis of fungal conidia (spores) by human phagocytes. Here, we present a fast and flexible way to analyze phagocytosis by flow cytometry using fluorescein isothiocyanate (FITC) labeling of conidia prior to co‐incubation with human leukocytes and an anti‐FITC counterstaining step postincubation to allow the discrimination of internalized and adherent conidia. In contrast to many other protocols, this method can be combined with further surface marker analyses. We sought to determine phagocytosis rates of <jats:styled-content style="fixed-case"><jats:italic>A. fumigatus</jats:italic></jats:styled-content> conidia in different stages and after several incubation times using this method. Moreover, we provide an example of application by comparing phagocytosis of <jats:styled-content style="fixed-case"><jats:italic>A. fumigatus</jats:italic></jats:styled-content> mutants to the wild type. © 2018 International Society for Advancement of Cytometry</jats:p>
  • Zugangsstatus: Freier Zugang