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Pinzon‐Olejua, Alejandro; Welte, Cornelia; Chekuru, Avinash; Bosak, Viktoria; Brand, Michael; Hans, Stefan; Stuermer, Claudia A.O.

Cre‐inducible site‐specific recombination in zebrafish oligodendrocytes

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  • Medientyp: E-Artikel
  • Titel: Cre‐inducible site‐specific recombination in zebrafish oligodendrocytes
  • Beteiligte: Pinzon‐Olejua, Alejandro; Welte, Cornelia; Chekuru, Avinash; Bosak, Viktoria; Brand, Michael; Hans, Stefan; Stuermer, Claudia A.O.
  • Erschienen: Wiley, 2017
  • Erschienen in: Developmental Dynamics, 246 (2017) 1, Seite 41-49
  • Sprache: Englisch
  • DOI: 10.1002/dvdy.24458
  • ISSN: 1058-8388; 1097-0177
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: Background:The conditional Cre/lox system has recently emerged as a valuable tool for studies on both embryonic and adult Zebrafish. Temporal control and site‐specific recombination are achieved by using the ligand‐inducible CreERT2and administration of the drug tamoxifen (TAM) or its active metabolite, 4‐Hydroxytamoxifen (4‐OHT).Results:Here we report the generation of a transgenic Zebrafish line, which expresses an mCherry‐tagged variant of CreERT2under the control of themyelin basic protein a(mbpa) promoter. Our analysis shows that larval and adult expression of the transgene recapitulates the endogenousmbpaexpression pattern in oligodendrocytes. Furthermore, combination with a Cre‐dependent EGFP reporter results in EGFP‐expressing oligodendrocytes in the spinal cord, brain, and optic nerve in TAM‐ or 4‐OHT‐treated larvae and 4‐month‐old fish, but not in untreated controls.Conclusions:The transgenic Zebrafish lineTg(mbpa:mCherry‐T2A‐CreERT2)elicitsCreERT2expression specifically in myelinating glia cells. Cre‐inducible targeted recombination of genes in oligodendrocytes will be useful to elucidate cellular and molecular mechanisms of myelination in vivo during development (myelination) and regeneration (remyelination) after injury to the central nervous system (CNS). It will also allow targeted expression and overexpression of genes of interest (transgenes) in oligodendrocytes at defined developmental and adult stages.Developmental Dynamics 246:41–49, 2017. © 2016 Wiley Periodicals, Inc.
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