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Medientyp: E-Artikel Titel: Expression of glucose phosphate isomerase in interspecific hybrid (Mus musculus × Mus caroli) Beteiligte: West, John D.; Ansell, John D.; Flockhart, Jean H. Erschienen: Wiley, 1991 Erschienen in: Developmental Genetics Sprache: Englisch DOI: 10.1002/dvg.1020120605 ISSN: 0192-253X; 1520-6408 Schlagwörter: Cell Biology ; Developmental Biology ; Genetics Entstehung: Anmerkungen: Beschreibung: <jats:title>Abstract</jats:title><jats:p>Hybrid <jats:italic>Mus musculus</jats:italic> × <jats:italic>Mus caroli</jats:italic> embryos were produced by inseminating <jats:italic>M. musculus</jats:italic> (C57BL/Ola Ws) females with <jats:italic>M. caroli</jats:italic> sperm. Control <jats:italic>M. caroli</jats:italic> embryos developed more rapidly than did control <jats:italic>M. musculus</jats:italic> embryos and implanted approximately 1 day earlier. At 1 1/2 days, both the hybrid embryos and those of the maternal species (<jats:italic>M. musculus</jats:italic>) had cleaved to the 2‐cell stage. By 2 1/2 days some of the hybrids were retarded compared to <jats:italic>M. musculus</jats:italic>, and by 3½ days most were lagging behind. This is consistent with the idea that the rate of development of hybrid embryos declines once it becomes dependent on embryo‐coded gene products.</jats:p><jats:p>We have used this difference in rate of preim‐plantation development, between hybrid and <jats:italic>M. musculus</jats:italic> embryos, to try to determine whether the activation of embryonic <jats:italic>Gpi</jats:italic>‐<jats:italic>1s</jats:italic> genes, that encode glucose phosphate isomerase (GPI‐1), is age‐related or stage‐related. In control <jats:italic>M. musculus</jats:italic> embryos (both mated and Al groups), the GPI‐1AB and GPI‐1A allozyme, indicative of paternal gene expression, were detected in 7 of 9 samples of 3 1/2‐day compacted morula stage embryos and were seen in all 19 samples of 31/2‐day blastocysts. In hybrid embryos, these allozymes were detected 1 day later. They were not detected in any 31/2‐day samples (12 samples of compacted morulae) but were consistently detected at 4½ days (4 samples of blastocysts and 2 samples of uncompacted morulae). Our interpretation of the results is that gene activation in hybrid embryos is stage‐specific, rather than age‐specific, and probably begins around the 8‐cell stage, with detectable levels of enzyme accumulating later. Analysis of GPI‐1 elec‐trophoresis indicated that both the paternal <jats:italic>(M. caroli)</jats:italic> and maternal <jats:italic>(M. musculus)</jats:italic> <jats:italic>Gpi</jats:italic>‐<jats:italic>1s</jats:italic> alleles were equally expressed in hybrid embryos and that the paternally derived allele was not activated before the maternally derived allele. © 1992 Wiley‐Liss, Inc.</jats:p>