Beschreibung:
<jats:title>Abstract</jats:title><jats:p>This paper describes the development of an amperometric biosensor based on galactose oxidase (GAOx) immobilization within a laponite clay film deposited on Carbon Screen‐Printed Electrodes modified by electrodeposited Prussian Blue and coated with poly‐(<jats:italic>O</jats:italic>‐phenylenediamine) (PPD/PB/CSPEs). Amperometric performances of GAOx@laponite/PPD/PB/CSPEs bioelectrodes were determined using several GAOx substrates. Using these modified electrodes the reduction of enzymatically generated hydrogen peroxide was performed at −0.2 V vs. Ag‐AgCl. In an initial attempt, <jats:italic>E.Coli</jats:italic> transketolase activity on its immobilized form was followed using a bienzymatic GAOx‐TK biosensor.</jats:p>