Leigh Anderson, N.;
Nance, Sharron L.;
Tollaksen, Sandra L.;
Giere, Frederic A.;
Anderson, Norman G.
Quantitative reproducibility of measurements from Coomassie Blue‐stained two‐dimensional gels: Analysis of mouse liver protein patterns and a comparison of BALB/c and C57 strains
Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Quantitative reproducibility of measurements from Coomassie Blue‐stained two‐dimensional gels: Analysis of mouse liver protein patterns and a comparison of BALB/c and C57 strains
Beteiligte:
Leigh Anderson, N.;
Nance, Sharron L.;
Tollaksen, Sandra L.;
Giere, Frederic A.;
Anderson, Norman G.
Beschreibung:
<jats:title>Abstract</jats:title><jats:p>Using the ISO‐DALT system for two‐dimensional (2‐D) electrophoresis and the TYCHO system for computer analysis of the resulting protein maps, we obtained high quality quantitative protein abundance data from Coomassie Brilliant Bluestained gels of mouse liver samples. High resolution gels allow more than 100 proteins to be measured with coefficients of variation less than 15 %. A comparison of results from two mouse strains (C57BL/6 and BALB/c) and the cross between them (BCF<jats:sub>1</jats:sub>) shows that a large number of qutive polymorphisms can be detected, and that, as expected, the amount of protein produced in the heterozygote is intermediate between the parental values. The system described is shown to be capable of reliably detecting decreases in protein abundance such as those expected to result from radiation‐induced deletion of one copy of a gene. The implications of these results for the study of gene regulation are discussed in relation to applications in genetics, toxicology, and differentiation.</jats:p>