• Medientyp: E-Artikel
  • Titel: The influence of proteins on silver staining of nucleic acids following polyacrylamide gel electrophoresis
  • Beteiligte: Hilbert, Friederike; Mayr, Burkhard; Lackner, Fritz; Bauer, Friedrich
  • Erschienen: Wiley, 1995
  • Erschienen in: ELECTROPHORESIS
  • Sprache: Englisch
  • DOI: 10.1002/elps.11501601152
  • ISSN: 0173-0835; 1522-2683
  • Schlagwörter: Clinical Biochemistry ; Biochemistry ; Analytical Chemistry
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>The influence, on silver staining, of proteins and restriction enzymes in polymerase chain reaction (PCR) products was studied in 12–20% polyacrylamide gels. For small DNA fragments (74, 41 and 33 bp) best results were achieved using 20% polyacrylamide gels. In 12% gels, restriction enzymes and also proteins used for PCR (bovine serum albumin, <jats:italic>Taq</jats:italic>‐DNA polymerase) interfere with silver staining of nucleic acids.</jats:p>