Novel tumor antigens identified by autologous antibody screening of childhood medulloblastoma cDNA libraries

Medientyp: E-Artikel
Titel: Novel tumor antigens identified by autologous antibody screening of childhood medulloblastoma cDNA libraries
Beteiligte: Behrends, Uta; Schneider, Inken; Rössler, Sabine; Frauenknecht, Heinrich; Golbeck, Anja; Lechner, Brigitte; Eigenstetter, Gerhard; Zobywalski, Colette; Müller‐Weihrich, Stephan; Graubner, Ulrike; Schmid, Irene; Sackerer, Dieter; Späth, Manfred; Goetz, Claudia; Prantl, Franz; Asmuss, Hans‐Peter; Bise, Karl; Mautner, Josef
Erschienen: Wiley, 2003
Erschienen in: International Journal of Cancer
Sprache: Englisch
DOI: 10.1002/ijc.11208
ISSN: 0020-7136; 1097-0215
Schlagwörter: Cancer Research ; Oncology
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Beschreibung: <jats:title>Abstract</jats:title><jats:p>Medulloblastoma is an embryonal childhood malignancy with poor prognosis. By screening 4 medulloblastoma cDNA expression libraries (SEREX) with autologous sera, 15 different antigens were identified. These antigens were encoded by 3 novel genes, genes of unknown function (KIAA0445, KIAA1853, KIAA0665, FLJ13942, HSPC213), a proto‐oncogene (rab18), candidate tumor suppressor genes (BAP1, PRDM13) and genes encoding a motor protein (kinesin‐2), a histone (H2A1.2), the ankyrin residue‐rich nasopharyngeal cancer susceptibility protein (NZ16) and the transcription factor TZP, which is homologous to the tumor‐associated antigens HCA58 and GLEA2. In a consecutive analysis of serum antibody titers and tumor load, a more than 10‐fold increase in serum antibodies against PRDM13 preceded the clinical diagnosis of recurrent tumor growth in a patient with aggressive large cell medulloblastoma. When sera of pediatric patients with cancer (<jats:italic>n</jats:italic> = 40) and healthy controls (<jats:italic>n</jats:italic> = 40) were tested for humoral responses against the SEREX‐defined antigens, 5 antigens were exclusively recognized by sera from cancer patients. These antigens included a novel rab18 gene product translated from mRNA sequences formerly described as 3′ untranslated region. Humoral responses against 2 of the remaining 10 antigens were found preferentially in cancer patients. Antibodies against these antigens were detected in 8/40 and 12/40 cancer patients, respectively, but in only 1 healthy control. The 2 antigens were characterized by a tumor‐specific deletion and a tumor‐specific mutation, respectively. These findings indicate that the humoral immune response against medulloblastoma is directed against diverse antigens that may be useful as diagnostic markers or targets for immunotherapy. © 2003 Wiley‐Liss, Inc.</jats:p>
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