Beschreibung:
<jats:title>Abstract</jats:title><jats:p>Many diseases can overrule natural pH regulatory mechanisms and alter the extracellular pH (pH<jats:sub>e</jats:sub>). A non‐invasive method that resolves pH<jats:sub>e</jats:sub> <jats:italic>in vivo</jats:italic> with high spatial and temporal resolution could therefore improve diagnosis and monitoring of diseases, contributing to the concept of precision medicine. During the last decades, several techniques have been proposed to image pH<jats:sub>e</jats:sub> non‐invasively. The majority of these methods rely on magnetic resonance because of its good spatial resolution, high penetration depth, non‐ionizing radiation and excellent complimentary soft tissue contrast. Dissolution dynamic nuclear polarization (DNP) is an emerging concept to enhance nuclear magnetic resonance (NMR) signals by more than four orders of magnitude, making it possible to observe <jats:italic>in vivo</jats:italic> metabolic processes in real‐time. Here, we summarize and review recent developments in pH<jats:sub>e</jats:sub> imaging techniques based on hyperpolarization methods and give an overview of recently discovered hyperpolarized pH sensor molecules that have been applied <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic>.</jats:p>