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Medientyp: E-Artikel Titel: Kinetics of TmHU binding to DNA as observed by optical tweezers Beteiligte: Salomo, Mathias; Keyser, Ulrich F.; Kegler, Kati; Gutsche, Christof; Struhalla, Marc; Immisch, Claudia; Hahn, Ulrich; Kremer, Friedrich Erschienen: Wiley, 2007 Erschienen in: Microscopy Research and Technique, 70 (2007) 11, Seite 938-943 Sprache: Englisch DOI: 10.1002/jemt.20498 ISSN: 1097-0029; 1059-910X Schlagwörter: Medical Laboratory Technology ; Instrumentation ; Histology ; Anatomy Entstehung: Anmerkungen: Beschreibung: AbstractThe kinetics of binding for the histone‐like protein TmHU (from Thermotoga maritima) to DNA is analyzed on a single molecule level by use of optical tweezers. For the reaction rate a pronounced concentration‐dependence is found with an “all or nothing”‐limit which suggests the cooperative nature of the binding‐reaction. By analyzing the statistics of mechanically induced dissociation‐events of TmHU from DNA multiple reaction sites are observed to become more likely with increasing TmHU concentration. This is interpreted as a hint for a secondary organizational level of the TmHU/DNA complex. The reaction rate of TmHU binding to DNA is remarkably higher than that of the HU protein from Escherichia coli which will be discussed. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.