> Detailanzeige

Ben Ami Pilo, Hila; Khan Khilji, Sana; Lühle, Jost; Biskup, Karina; Levy Gal, Bar; Rosenhek Goldian, Irit; Alfandari, Daniel; Revach, Or‐Yam; Kiper, Edo; Morandi, Mattia I.; Rotkopf, Ron; Porat, Ziv; Blanchard, Véronique; Seeberger, Peter H.; Regev‐Rudzki, Neta; Moscovitz, Oren

Sialylated N‐glycans mediate monocyte uptake of extracellular vesicles secreted from Plasmodium falciparum‐infected red blood cells

Literatur-
verwaltung

Zur
Merkliste

Lösche von
Merkliste

Per Whatsapp teilen

Schließen

Merkliste



Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
  • Medientyp: E-Artikel
  • Titel: Sialylated N‐glycans mediate monocyte uptake of extracellular vesicles secreted from Plasmodium falciparum‐infected red blood cells
  • Beteiligte: Ben Ami Pilo, Hila; Khan Khilji, Sana; Lühle, Jost; Biskup, Karina; Levy Gal, Bar; Rosenhek Goldian, Irit; Alfandari, Daniel; Revach, Or‐Yam; Kiper, Edo; Morandi, Mattia I.; Rotkopf, Ron; Porat, Ziv; Blanchard, Véronique; Seeberger, Peter H.; Regev‐Rudzki, Neta; Moscovitz, Oren
  • Erschienen: Wiley, 2022
  • Erschienen in: Journal of Extracellular Biology
  • Sprache: Englisch
  • DOI: 10.1002/jex2.33
  • ISSN: 2768-2811
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>Glycoconjugates on extracellular vesicles (EVs) play a vital role in internalization and mediate interaction as well as regulation of the host immune system by viruses, bacteria, and parasites. During their intraerythrocytic life‐cycle stages, malaria parasites, <jats:italic>Plasmodium falciparum</jats:italic> (<jats:italic>Pf</jats:italic>) mediate the secretion of EVs by infected red blood cells (RBCs) that carry a diverse range of parasitic and host‐derived molecules. These molecules facilitate parasite‐parasite and parasite‐host interactions to ensure parasite survival.</jats:p><jats:p>To date, the number of identified <jats:italic>Pf</jats:italic> genes associated with glycan synthesis and the repertoire of expressed glycoconjugates is relatively low. Moreover, the role of <jats:italic>Pf</jats:italic> glycans in pathogenesis is mostly unclear and poorly understood. As a result, the expression of glycoconjugates on <jats:italic>Pf</jats:italic>‐derived EVs or their involvement in the parasite life‐cycle has yet to be reported.</jats:p><jats:p>Herein, we show that EVs secreted by <jats:italic>Pf</jats:italic>‐infected RBCs carry significantly higher sialylated complex <jats:italic>N</jats:italic>‐glycans than EVs derived from healthy RBCs. Furthermore, we reveal that EV uptake by host monocytes depends on N‐glycoproteins and demonstrate that terminal sialic acid on the <jats:italic>N</jats:italic>‐glycans is essential for uptake by human monocytes. Our results provide the first evidence that <jats:italic>Pf</jats:italic> exploits host sialylated <jats:italic>N</jats:italic>‐glycans to mediate EV uptake by the human immune system cells.</jats:p>
  • Zugangsstatus: Freier Zugang