Endocytic uptake of fluorescence labelled DNA in yeast

Medientyp: E-Artikel
Titel: Endocytic uptake of fluorescence labelled DNA in yeast
Beteiligte: Riechers, Sean‐Patrick; Stahl, Ulf; Lang, Christine
Erschienen: Wiley, 2010
Erschienen in: Journal of Basic Microbiology
Sprache: Englisch
DOI: 10.1002/jobm.200900279
ISSN: 0233-111X; 1521-4028
Schlagwörter: Applied Microbiology and Biotechnology ; General Medicine
Entstehung:
Anmerkungen:
Beschreibung: <jats:title>Abstract</jats:title><jats:p>After dispiriting results using viral vectors in gene therapy, by which a number of patients acquired cancer as a result of the use of retroviral vector constructs, the percentage of non‐viral approaches has increased over recent years. To elucidate potential bottlenecks in the non‐viral transfection process we here introduce a novel method to directly visualize endocytic non‐viral DNA uptake in a transfection approach. This novel method allows for the first time to monitor the location of DNA which is taken up by endocytosis in yeast (<jats:italic>Saccharomyces cerevisiae</jats:italic>) wild type and mutant strains. More specifically it enables drawing conclusions about conditions favouring non‐viral gene transfection. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)</jats:p>

Nur in Feld suchen: