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Medientyp: E-Artikel Titel: Screening of l‐histidine‐based ligands to modify monolithic supports and selectively purify the supercoiled plasmid DNA isoform Beteiligte: Amorim, Lúcia F. A.; Sousa, Fani; Queiroz, João A.; Cruz, Carla; Sousa, Ângela Erschienen: Wiley, 2015 Erschienen in: Journal of Molecular Recognition, 28 (2015) 6, Seite 349-358 Sprache: Englisch DOI: 10.1002/jmr.2449 ISSN: 0952-3499; 1099-1352 Schlagwörter: Molecular Biology ; Structural Biology Entstehung: Anmerkungen: Beschreibung: The growing demand of pharmaceutical‐grade plasmid DNA (pDNA) suitable for biotherapeutic applications fostered the development of new purification strategies. The surface plasmon resonance technique was employed for a fast binding screening of l‐histidine and its derivatives, 1‐benzyl‐l‐histidine and 1‐methyl‐l‐histidine, as potential ligands for the biorecognition of three plasmids with different sizes (6.05, 8.70, and 14 kbp). The binding analysis was performed with different isoforms of each plasmid (supercoiled, open circular, and linear) separately. The results revealed that the overall affinity of plasmids to l‐histidine and its derivatives was high (KD > 10−8 M), and the highest affinity was found for human papillomavirus 16 E6/E7 (KD = 1.1 × 10−10 M and KD = 3.34 × 10−10 M for open circular and linear plasmid isoforms, respectively). l‐Histidine and 1‐benzyl‐l‐histidine were immobilized on monolithic matrices. Chromatographic studies of l‐histidine and 1‐benzyl‐l‐histidine monoliths were also performed with the aforementioned samples. In general, the supercoiled isoform had strong interactions with both supports. The separation of plasmid isoforms was achieved by decreasing the ammonium sulfate concentration in the eluent, in both supports, but a lower salt concentration was required in the 1‐benzyl‐l‐histidine monolith because of stronger interactions promoted with pDNA. The efficiency of plasmid isoforms separation remained unchanged with flow rate variations. The binding capacity for pDNA achieved with the l‐histidine monolith was 29‐fold higher than that obtained with conventional l‐histidine agarose. Overall, the combination of either l‐histidine or its derivatives with monolithic supports can be a promising strategy to purify the supercoiled isoform from different plasmids with suitable purity degree for pharmaceutical applications. Copyright © 2015 John Wiley & Sons, Ltd.