Synthesis and identification of hydroxylated metabolites of the anti‐estrogenic agent cyclofenil

Medientyp: E-Artikel
Titel: Synthesis and identification of hydroxylated metabolites of the anti‐estrogenic agent cyclofenil
Beteiligte: Gärtner, Peter; Hofbauer, Karin; Reichel, Christian; Geisendorfer, Thomas; Gmeiner, Günter
Erschienen: Wiley, 2008
Erschienen in: Journal of Mass Spectrometry
Sprache: Englisch
DOI: 10.1002/jms.1462
ISSN: 1076-5174; 1096-9888
Schlagwörter: Spectroscopy
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Beschreibung: <jats:title>Abstract</jats:title><jats:p>The detection of metabolites of the anti‐estrogenic substance cyclofenil, listed on the World Anti‐Doping Agency (WADA) Prohibited List since 2004 is described. Target substances are hydroxylated metabolites, bearing an aliphatic hydroxyl group either in the 2‐, 3‐ or 4‐position of the aliphatic ring, in addition to the phenolic functions on the aromatic rings. Structural identification used NMR as well as high‐resolution mass spectrometry after nano‐electrospray ionisation (ESI). Unambiguous detection of all three synthesised cyclofenil metabolites M1M3 was done using gas chromatography for separation and electron ionisation mass spectrometry for detection of the per‐silylated compounds in comparison with a reference urine deriving from an excretion study within the WADA 2007 Educational Programme. Copyright © 2008 John Wiley & Sons, Ltd.</jats:p>

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