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Medientyp:
E-Artikel
Titel:
The Brassica epithionitrile 1‐cyano‐2,3‐epithiopropane triggers cell death in human liver cancer cells in vitro
Beteiligte:
Hanschen, Franziska S.;
Herz, Corinna;
Schlotz, Nina;
Kupke, Franziska;
Bartolomé Rodríguez, María M.;
Schreiner, Monika;
Rohn, Sascha;
Lamy, Evelyn
Erschienen:
Wiley, 2015
Erschienen in:Molecular Nutrition & Food Research
Sprache:
Englisch
DOI:
10.1002/mnfr.201500296
ISSN:
1613-4125;
1613-4133
Entstehung:
Anmerkungen:
Beschreibung:
<jats:sec><jats:title>Scope</jats:title><jats:p>Glucosinolates are secondary metabolites present in <jats:italic>Brassica</jats:italic> vegetables. Alkenyl glucosinolates are enzymatically degraded forming nitriles or isothiocyanates, but in the presence of epithiospecifier protein, epithionitriles are released. However, studies on the occurrence of epithionitriles in <jats:italic>Brassica</jats:italic> food and knowledge about their biological effects are scarce.</jats:p></jats:sec><jats:sec><jats:title>Methods and results</jats:title><jats:p>Epithionitrile formation from glucosinolates of seven <jats:italic>Brassica</jats:italic> vegetables was analyzed using GC‐MS and HPLC‐DAD. Bioactivity of synthetic and plant‐derived 1‐cyano‐2,3‐epithiopropane (CETP) – the predominant epithionitrile in <jats:italic>Brassica</jats:italic> vegetables – in three human hepatocellular carcinoma (HCC) cell lines and primary murine hepatocytes was also evaluated. The majority of the <jats:italic>Brassica</jats:italic> vegetables were producers of nitriles or epithionitriles as hydrolysis products and not of isothiocyanates. For example, Brussels sprouts and savoy cabbage contained up to 0.8 μmol CETP/g vegetable. Using formazan dye assays, concentrations of 380–1500 nM CETP were observed to inhibit the mitochondrial dehydrogenase activity of human HCC cells without impairment of cell growth. At 100‐fold higher CETP concentrations, cell death was observed. Presence of plant matrix increased CETP‐based toxicity.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>These in vitro data provide no indication that epithionitriles will severely affect human health by <jats:italic>Brassica</jats:italic> consumption. In contrast to isothiocyanates, no evidence of selective toxicity against HCC cells was found.</jats:p></jats:sec>