Beteiligte:
Quiala, Elisa;
Cañal, María Jesús;
Rodríguez, Roberto;
Yagüe, Norma;
Chávez, Maité;
Barbón, Raúl;
Valledor, Luis
Erschienen:
Wiley, 2012
Erschienen in:
PROTEOMICS, 12 (2012) 7, Seite 1039-1044
Sprache:
Englisch
DOI:
10.1002/pmic.201100183
ISSN:
1615-9853;
1615-9861
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p><jats:italic><jats:styled-content style="fixed-case">T</jats:styled-content>ectona grandis</jats:italic><jats:styled-content style="fixed-case">L</jats:styled-content>. (teak) is one of the premier hardwood timbers in the world, ranking at present in the top five tropical hardwood species in terms of worldwide plantation area. Characterization of the proteins present in teak leaves will provide a basis for the development of new tools aimed at assisting tree selection, the monitoring of plant propagation, and the certification of clonal and phenotypic identities. In this paper, we describe the extraction, separation, and identification of leaf proteins from<jats:italic><jats:styled-content style="fixed-case">T</jats:styled-content>. grandis</jats:italic>using a<jats:styled-content style="fixed-case">TCA</jats:styled-content>/acetone protocol, 2DE, and MALDI‐TOF. After<jats:styled-content style="fixed-case">TCA</jats:styled-content>/acetone protein extraction of leaves, 998 well‐resolved spots were detected in Coomassie‐stained gels within the 10–114 k<jats:styled-content style="fixed-case">D</jats:styled-content>a relative molecular mass (<jats:styled-content style="fixed-case">M</jats:styled-content><jats:italic>r</jats:italic>) range at a p<jats:styled-content style="fixed-case">H</jats:styled-content>ranging from 3 to 11. A total of 120 spots were digested and subjected to<jats:styled-content style="fixed-case">MS</jats:styled-content>. Of these, 100 nonredundant protein species were successfully identified. Functional classification of the identified proteins revealed that proteins involved in photosynthesis, protein translation, and energy production were the most abundant. This work is the first high‐throughput attempt to study the<jats:italic><jats:styled-content style="fixed-case">T</jats:styled-content>. grandis</jats:italic>leaf proteome and represents a stepping stone for further differential expression proteomic studies related to growth, development, biomass production, and culture‐associated physiological responses.</jats:p>