> Merkliste Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp: E-Artikel Titel: Biochemical evidence that an S431F mutation in acetylcholinesterase‐1 of Aphis gossypii mediates resistance to pirimicarb and omethoate Beteiligte: Benting, Jürgen; Nauen, Ralf Erschienen: Wiley, 2004 Erschienen in: Pest Management Science Sprache: Englisch DOI: 10.1002/ps.971 ISSN: 1526-498X; 1526-4998 Schlagwörter: Insect Science ; Agronomy and Crop Science ; General Medicine Entstehung: Anmerkungen: Beschreibung: <jats:title>Abstract</jats:title><jats:p>Molecular changes in acetylcholinesterase (AChE) leading to target‐site resistance to carbamate and organophosphate insecticides have recently been identified. Of particular interest is the S431F mutation in <jats:italic>ace2</jats:italic> and its orthologue <jats:italic>ace1</jats:italic> of <jats:italic>Myzus persicae</jats:italic> Sulzer and <jats:italic>Aphis gossypii</jats:italic> Glover, respectively. This mutation has been correlated with resistance to pirimicarb, but biochemical evidence has not yet been provided. Here, we describe for the first time that recombinantly expressed AChE1 from <jats:italic>A gossypii</jats:italic> carrying the S431F mutation is insensitive to pirimicarb and omethoate, but sensitive to demeton‐S‐methyl and hypersensitive to carbofuran. Furthermore, site‐directed mutagenesis of the serine residue at position 431 in <jats:italic>ace1</jats:italic> from a pirimicarb‐susceptible clone of <jats:italic>A gossypii</jats:italic> conferred insensitivity to pirimicarb. We conclude that AChE1 of <jats:italic>A gossypii</jats:italic> is the target of toxicological relevance of carbamates and organophosphates. Copyright © 2004 Society of Chemical Industry</jats:p>