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Marshall, Ron; Hanley, Steven J; Hull, Richard; Moss, Stephen R

The presence of two different target‐site resistance mechanisms in individual plants of Alopecurus myosuroides Huds., identified using a quick molecular test for the characterisation of six ALS and seven ACCase SNPs

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  • Medientyp: E-Artikel
  • Titel: The presence of two different target‐site resistance mechanisms in individual plants of Alopecurus myosuroides Huds., identified using a quick molecular test for the characterisation of six ALS and seven ACCase SNPs
  • Beteiligte: Marshall, Ron; Hanley, Steven J; Hull, Richard; Moss, Stephen R
  • Erschienen: Wiley, 2013
  • Erschienen in: Pest Management Science
  • Sprache: Englisch
  • DOI: 10.1002/ps.3429
  • ISSN: 1526-498X; 1526-4998
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:sec><jats:title>BACKGROUND</jats:title><jats:p><jats:bold>Target‐site resistance to <jats:styled-content style="fixed-case">ALS</jats:styled-content>‐ and <jats:styled-content style="fixed-case">ACCase</jats:styled-content>‐inhibiting herbicides in the grass weed <jats:italic>Alopecurus myosuroides</jats:italic> is associated with well‐characterised allelic variants encoding <jats:styled-content style="fixed-case">ALS</jats:styled-content>‐ and <jats:styled-content style="fixed-case">ACCase</jats:styled-content>‐based resistance. The potential for combined <jats:styled-content style="fixed-case">ALS</jats:styled-content> and <jats:styled-content style="fixed-case">ACCase</jats:styled-content> resistance presents a threat to future control, given the extent to which these herbicides are used. The authors present a primer extension method for rapid detection of known resistance‐conferring substitutions.</jats:bold></jats:p></jats:sec><jats:sec><jats:title>RESULTS</jats:title><jats:p><jats:bold>Individuals showing combined resistance to field‐rate mesosulfuron + iodosulfuron and cycloxydim were identified in four field‐collected populations, with proportions ranging from 30 to 100%. Genotyping with the <jats:styled-content style="fixed-case">SNaPshot</jats:styled-content> primer extension kit showed the <jats:styled-content style="fixed-case">T197</jats:styled-content> and <jats:styled-content style="fixed-case">L574 ALS</jats:styled-content> and <jats:styled-content style="fixed-case">L1781 ACCase</jats:styled-content> isoforms to be associated with <jats:styled-content style="fixed-case">ALS</jats:styled-content> and <jats:styled-content style="fixed-case">ACCase</jats:styled-content> resistance whenever they occurred.</jats:bold></jats:p></jats:sec><jats:sec><jats:title>CONCLUSION</jats:title><jats:p><jats:bold>Combined <jats:styled-content style="fixed-case">ALS</jats:styled-content> and <jats:styled-content style="fixed-case">ACCase</jats:styled-content> target‐site resistance threatens future control of <jats:italic>A. myosuroides</jats:italic>. The <jats:styled-content style="fixed-case">SNaPshot</jats:styled-content> extension assay provides a reliable new multiplexable method for characterising known allelic variants of the <jats:styled-content style="fixed-case">ALS</jats:styled-content> and <jats:styled-content style="fixed-case">ACCase</jats:styled-content> genes of <jats:italic>A. myosuroides</jats:italic>. The method offers significant advantages over both <jats:styled-content style="fixed-case">CAPS</jats:styled-content>/<jats:styled-content style="fixed-case">dCAPS</jats:styled-content> and <jats:styled-content style="fixed-case">PASA</jats:styled-content> in that full genotyping can be accomplished at any nucleotide position using a single extension primer. © 2012 Society of Chemical Industry</jats:bold></jats:p></jats:sec>