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Zhu, Xingyu; Sato, Toshinori

The distinction of underivatized monosaccharides using electrospray ionization ion trap mass spectrometry

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  • Medientyp: E-Artikel
  • Titel: The distinction of underivatized monosaccharides using electrospray ionization ion trap mass spectrometry
  • Beteiligte: Zhu, Xingyu; Sato, Toshinori
  • Erschienen: Wiley, 2007
  • Erschienen in: Rapid Communications in Mass Spectrometry
  • Sprache: Englisch
  • DOI: 10.1002/rcm.2825
  • ISSN: 0951-4198; 1097-0231
  • Schlagwörter: Organic Chemistry ; Spectroscopy ; Analytical Chemistry
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>A convenient method for distinguishing underivatized isomeric monosaccharides has been established using electrospray ionization ion trap mass spectrometry (ESI‐ITMS). Mass spectra of hexoses (glucose, galactose, and mannose),<jats:italic>N</jats:italic>‐acetylhexosamines (<jats:italic>N</jats:italic>‐acetylglucosamine,<jats:italic>N</jats:italic>‐acetylgalactosamine, and<jats:italic>N</jats:italic>‐acetylmannosamine) and hexosamines (glucosamine, galactosamine, and mannosamine) dissolved in solvent containing 1 mM ammonium acetate were obtained in the positive ion mode. Glucose was distinguished from galactose and mannose in the MS<jats:sup>2</jats:sup>spectrum of the [M+NH<jats:sub>4</jats:sub>]<jats:sup>+</jats:sup>ion at<jats:italic>m/z</jats:italic>198. The MS<jats:sup>3</jats:sup>spectra generated from [M+NH<jats:sub>4</jats:sub>–H<jats:sub>2</jats:sub>O–NH<jats:sub>3</jats:sub>]<jats:sup>+</jats:sup>at<jats:italic>m/z</jats:italic>163 showed that galactose and mannose could be distinguished by the ratio of peak intensities at<jats:italic>m/z</jats:italic>145 and 127, while the three<jats:italic>N</jats:italic>‐acetylhexosamine and hexosamine stereochemical isomers could be identified by the relative abundance ratios of product ions observed in MS<jats:sup>3</jats:sup>spectra. The investigation of MS and MS<jats:sup>2</jats:sup>spectra from complexes of these monosaccharides with Na<jats:sup>+</jats:sup>and Pb<jats:sup>2+</jats:sup>failed to distinguish these monosaccharide isomers. Therefore, multiple stage mass analysis by ESI‐ITMS using either [M+NH<jats:sub>4</jats:sub>]<jats:sup>+</jats:sup>or [M+H]<jats:sup>+</jats:sup>was useful to distinguish between the isomers of monosaccharides. Copyright © 2006 John Wiley &amp; Sons, Ltd.</jats:p>