Clot, Corentin R.;
Vexler, Lea;
de La O Leyva-Perez, Maria;
Bourke, Peter M.;
Engelen, Christel J. M.;
Hutten, Ronald C. B.;
van de Belt, José;
Wijnker, Erik;
Milbourne, Dan;
Visser, Richard G. F.;
Juranić, Martina;
van Eck, Herman J.
Identification of two mutant JASON-RELATED genes associated with unreduced pollen production in potato
Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Identification of two mutant JASON-RELATED genes associated with unreduced pollen production in potato
Beteiligte:
Clot, Corentin R.;
Vexler, Lea;
de La O Leyva-Perez, Maria;
Bourke, Peter M.;
Engelen, Christel J. M.;
Hutten, Ronald C. B.;
van de Belt, José;
Wijnker, Erik;
Milbourne, Dan;
Visser, Richard G. F.;
Juranić, Martina;
van Eck, Herman J.
Erschienen:
Springer Science and Business Media LLC, 2024
Erschienen in:Theoretical and Applied Genetics
Sprache:
Englisch
DOI:
10.1007/s00122-024-04563-7
ISSN:
0040-5752;
1432-2242
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>Abstract</jats:title><jats:sec>
<jats:title>Key message</jats:title>
<jats:p>Multiple QTLs control unreduced pollen production in potato. Two major-effect QTLs co-locate with mutant alleles of genes with homology to AtJAS, a known regulator of meiotic spindle orientation.</jats:p>
</jats:sec><jats:sec>
<jats:title>Abstract</jats:title>
<jats:p>In diploid potato the production of unreduced gametes with a diploid (2<jats:italic>n</jats:italic>) rather than a haploid (<jats:italic>n</jats:italic>) number of chromosomes has been widely reported. Besides their evolutionary important role in sexual polyploidisation, unreduced gametes also have a practical value for potato breeding as a bridge between diploid and tetraploid germplasm. Although early articles argued for a monogenic recessive inheritance, the genetic basis of unreduced pollen production in potato has remained elusive. Here, three diploid full-sib populations were genotyped with an amplicon sequencing approach and phenotyped for unreduced pollen production across two growing seasons. We identified two minor-effect and three major-effect QTLs regulating this trait. The two QTLs with the largest effect displayed a recessive inheritance and an additive interaction. Both QTLs co-localised with genes encoding for putative <jats:italic>AtJAS</jats:italic> homologs, a key regulator of meiosis II spindle orientation in <jats:italic>Arabidopsis thaliana</jats:italic>. The function of these candidate genes is consistent with the cytological phenotype of mis-oriented metaphase II plates observed in the parental clones. The alleles associated with elevated levels of unreduced pollen showed deleterious mutation events: an exonic transposon insert causing a premature stop, and an amino acid change within a highly conserved domain. Taken together, our findings shed light on the natural variation underlying unreduced pollen production in potato and will facilitate interploidy breeding by enabling marker-assisted selection for this trait.</jats:p>
</jats:sec>