Beschreibung:
<jats:title>Abstract</jats:title><jats:sec>
<jats:title>Background</jats:title>
<jats:p>Parietal epithelial cells are a heterogeneous population of cells located on Bowman’s capsule. These cells are known to internalize albumin with a still undetermined mechanism, although albumin has been shown to induce phenotypic changes in parietal epithelial cells. Proximal tubular cells are the main actors in albumin handling via the macromolecular complex composed by ClC-5, megalin, and cubilin. This study investigated the role of ClC-5, megalin, and cubilin in the parietal epithelial cells of kidney biopsies from proteinuric lupus nephritis patients and control subjects and identified phenotypical changes occurring in the pathological milieu.</jats:p>
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<jats:title>Methods</jats:title>
<jats:p>Immunohistochemistry and immunofluorescence analyses for ClC-5, megalin, cubilin, ANXA3, podocalyxin, CD24, CD44, HSA, and LTA marker were performed on 23 kidney biopsies from patients with Lupus Nephritis and 9 control biopsies (obtained from nephrectomies for renal cancer).</jats:p>
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<jats:title>Results</jats:title>
<jats:p>Two sub-populations of hypertrophic parietal epithelial cells ANXA3<jats:sup>+</jats:sup>/Podocalyxin<jats:sup>−</jats:sup>/CD44<jats:sup>−</jats:sup>, both expressing ClC-5, megalin, and cubilin and located at the tubular pole, were identified and characterized: the first one, CD24<jats:sup>+</jats:sup>/HSA<jats:sup>−</jats:sup>/LTA<jats:sup>−</jats:sup> had characteristics of human adult parietal epithelial multipotent progenitors, the second one, CD24<jats:sup>−</jats:sup>/LTA<jats:sup>+</jats:sup>/HSA<jats:sup>+</jats:sup> committed to become phenotypically proximal tubular cells. The number of glomeruli presenting hypertrophic parietal epithelial cells positive for ClC-5, megalin, and cubilin were significantly higher in lupus nephritis patients than in controls.</jats:p>
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<jats:title>Conclusions</jats:title>
<jats:p>Our results may provide further insight into the role of hypertrophic parietal epithelial cells located at the tubular pole and their possible involvement in protein endocytosis in lupus nephritis patients. These data also suggest that the presence of hypertrophic parietal epithelial cells in Bowman's capsule represents a potential resource for responding to protein overload observed in other glomerulonephritis.</jats:p>
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<jats:title>Graphical abstract</jats:title>
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