Beschreibung:
<jats:p>The recombinant plasmid pNRK 36, which represents the plasmids RSF 1010, a small multicopy plasmid of the incompatability group IncQ that confers resistance to streptomycin and sulfoamide to its host cells, and pKK‐ 223‐3, which contains the try‐lac (tac) promoter followed by a polylinker and a DNA segment containing the 5 S rRNA (rrn B) with the ribosomal RNA transcription terminators, was employed to transform <jats:italic>Pseudomonas putida</jats:italic> 2440 cells. The plasmid encoded 5 S rRNA from <jats:italic>Escherichia coli</jats:italic> was transcribed and processed properly in <jats:italic>P. putida</jats:italic> cells thus demonstrating for the first time the expression of a plasmid encoded ribosomal RNA in a heterologous system. The fact that the <jats:italic>E. coli</jats:italic> 5 S rRNA was not incorporated into assembled ribosomes suggests that the in vivo incorporation of 5 S rRNA into the 50 S ribosomal sub‐unit is closely linked to 23 S rRNA and/or ribosomal protein synthesis.</jats:p>