Beschreibung:
<jats:p>By use of a new assay method based on HPLC, GlcNAc‐transferase III, IV and V activities were determined in HL‐60 cells, differentiated HL‐60 cells and normal myeloid cells. Differentiation along the monocytic lineage with 1α,25‐dihydroxyvitamin D<jats:sub>3</jats:sub> resulted in increased GlcNAc‐transferase IV and decreased GlcNAc‐transferase III activity. Differentiation along the myeloid lineage with retinoic acid resulted in a decrease in GlcNAc‐transferase III activity. Although differentiated HL‐60 cells show a changed GlcNAc‐transferase pattern, they do not resemble normal myeloid cells. Macrophages and granulocytes are characterized by a very low level of GlcNAc‐transferase III activity whereas differentiated HL‐60 cells still contain this activity. This is the first demonstration of GlcNAc‐transferase IV and V activity in a human cell.</jats:p>