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Medientyp:
E-Artikel
Titel:
Calcyclin is a calcium and zinc binding protein
Beteiligte:
Filipek, Anna;
Heizmann, Claus W.;
Kúznicki, Jacek
Erschienen:
Wiley, 1990
Erschienen in:
FEBS Letters, 264 (1990) 2, Seite 263-266
Sprache:
Englisch
DOI:
10.1016/0014-5793(90)80263-i
ISSN:
0014-5793;
1873-3468
Entstehung:
Anmerkungen:
Beschreibung:
Calcyclin, a cell cycle regulated protein, was recently purified from Ehrlich ascites tumour (EAT) cells and shown to be a calcium binding protein. Here we show that calcyclin monomer and dimer also bind zinc ions. Zinc binding sites seem to be different from calcium binding sites since: preincubation with Ca2+ lacks effect on the binding of Zn2+, and Ca2+ (but not Zn2+) increases tyrosine fluorescence intensity. Binding of Zn2+ reduces the extent of the conformational changes induced by Ca2+, and seems to affect Ca2+‐binding. The data suggest that Ca2+‐ and Zn2+‐ might trigger the biological activity of calcyclin.