Beschreibung:
<jats:p>SNAP‐25 (synaptosomal‐associated protein 25 kDa) is a target for botulinum neurotoxins A and E, which both inhibit neurotransmitter release, and was recently identified together with syntaxin and synaptobrevin as receptors for NSF and α‐SNAP. We show that SNAP‐25 was enriched in the microsomal fraction from adrenal medulla, although the level of SNAP‐25 in adrenal medullary microsomes was about 20‐fold less than in brain microsomes. Immunocytochemistry confirmed the presence of SNAP‐25 in cultured chromaffin cells and showed plasma membrane staining. Using immunoprecipitation, we found that SNAP‐25 was present in a complex with syntaxin, synaptobrevin, synaptotagmin, NSF, α‐SNAP and other unidentified polypeptides. These data indicate that SNAP‐25 in chromaffin cells is present in a complex similar to that identified in brain.</jats:p>