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Medientyp:
E-Artikel
Titel:
The micelle to vesicle transition of lipids and detergents in the presence of a membrane protein: towards a rationale for 2D crystallization
Beteiligte:
Dolder, Max;
Engel, Andreas;
Zulauf, Martin
Erschienen:
Wiley, 1996
Erschienen in:FEBS Letters
Sprache:
Englisch
DOI:
10.1016/0014-5793(96)00180-9
ISSN:
0014-5793;
1873-3468
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>The assembly of two‐dimensional membrane protein crystals in the presence of lipids was analyzed with quasielastic light scattering and electron microscopy. Mixtures of detergent‐solubilized lipids and/or proteins were submitted to slow or rapid dilution while measuring the hydrodynamic radii of the aggregates. Lipids alone exhibited λ‐shaped dilution curves with intermediate rod‐shaped particles that converted into small vesicles. Depending on the protein‐protein and protein‐lipid interactions, detergent‐solubilized protein‐lipid mixtures showed a sharp transition from micelles to large, densely packed proteoliposomes. Electron microscopy revealed that formation of crystals occurred shortly after this phase transition.</jats:p>