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Medientyp:
E-Artikel
Titel:
Molecular basis for the substrate specificity of plant guanine nucleotide exchange factors for ROP
Beteiligte:
Fricke, Inka;
Berken, Antje
Erschienen:
Wiley, 2009
Erschienen in:
FEBS Letters, 583 (2009) 1, Seite 75-80
Sprache:
Englisch
DOI:
10.1016/j.febslet.2008.12.008
ISSN:
0014-5793;
1873-3468
Entstehung:
Anmerkungen:
Beschreibung:
Plant G proteins of the ROP/RAC family regulate cellular processes including cytoskeletal rearrangement in polar growth. Activation of the ROP molecular switch is triggered by guanine nucleotide exchange factors. Plant‐specific RopGEFs are exclusively active on ROPs despite their high homology to animal Rho proteins. Based on a sequence comparison of ROPs vs. animal Rho proteins together with structural data on distinct ROPs, we identified unique substrate determinants of RopGEF specificity by mutational analysis: asparagine 68 next to switch II, arginine 76 of a putative phosphorylation motif and the Rho insert are essential for substrate recognition by RopGEFs. These data also provide first evidence for a function of the Rho insert in interactions with GEFs.