Enhancement of lymphocyte proliferation induced by interleukin‐12 and anti‐interleukin‐10 in HIV‐infected patients during highly active antiretroviral therapy
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Medientyp:
E-Artikel
Titel:
Enhancement of lymphocyte proliferation induced by interleukin‐12 and anti‐interleukin‐10 in HIV‐infected patients during highly active antiretroviral therapy
Beschreibung:
Based on the potentially important role of IL‐10 and IL‐12 in the pathogenesis of HIV infection, we have examined the effect of highly active antiretroviral therapy (HAART) on the production of these two cytokines, and whether addition of IL‐12 or anti‐IL‐10 in vitro could improve the proliferative response in peripheral blood mononuclear cells (PBMC) from HIV‐infected patients during such therapy. Our findings are: (i) After initiating HAART there were no significant changes in PHA‐ or MAC‐PPD‐stimulated IL‐10 and IL‐12 levels in PBMC supernatants in the patient group as a whole. (ii) However, while a decline in IL‐10 synthesis was shown in patients with high baseline MAC‐PPD‐ and PHA‐stimulated IL‐10 levels, IL‐10 increased in patients with lower baseline levels. A similar pattern was seen for MAC‐PPD‐stimulated IL‐12 levels. (iii) Exogenously added IL‐12 and anti‐IL‐10 markedly and additively improved MAC‐PPD‐stimulated PBMC proliferation in vitro. Thus, a loss of cell‐mediated immune response exists in HIV‐infected patients also during apparently successful HAART and this can be significantly improved by addition of IL‐12 and anti‐IL‐10, at least in vitro. These results suggest that further exploration of both IL‐10 and IL‐12 as targets for immunomodulating therapy in HIV‐infected patients in addition to HAART might be important.