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Buchmann, Rainer; Hasilik, Andrej; Nunn, Martha E.; Van Dyke, Thomas E.; Lange, Dieter E.

PMN responses in chronic periodontal disease: evaluation by gingival crevicular fluid enzymes and elastase‐alpha‐1‐proteinase inhibitor complex

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  • Medientyp: E-Artikel
  • Titel: PMN responses in chronic periodontal disease: evaluation by gingival crevicular fluid enzymes and elastase‐alpha‐1‐proteinase inhibitor complex
  • Beteiligte: Buchmann, Rainer; Hasilik, Andrej; Nunn, Martha E.; Van Dyke, Thomas E.; Lange, Dieter E.
  • Erschienen: Wiley, 2002
  • Erschienen in: Journal of Clinical Periodontology, 29 (2002) 6, Seite 563-572
  • Sprache: Englisch
  • DOI: 10.1034/j.1600-051x.2002.290613.x
  • ISSN: 1600-051X; 0303-6979
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: AbstractObjectives: In the present trial, the hypothesis was examined that the local PMN responses in untreated and treated chronic periodontitis can be differentiated by gingival crevicular fluid lysosomal enzyme activities and elastase‐alpha‐1‐proteinase inhibitor complex.Methods: In nine subjects (average age 49.2 ± 7.1 years) with chronic periodontitis, clinical parameters and markers of the PMN‐derived inflammatory tissue response in gingival crevicular fluid (GCF) were assessed before and 6 months after surgical periodontal therapy. Myeloperoxidase (MPO), beta‐N‐acetyl‐hexosaminidase (beta‐NAH) and cathepsin D (CD) were analyzed as indicators of the PMN‐associated host tissue destruction, and elastase‐alpha‐1‐proteinase inhibitor complex (alpha‐1‐EPI) as the major serum protein inactivating PMN elastase. The total activities of the lysosomal enzymes MPO and beta‐NAH were evaluated spectrophotometrically, the CD levels by liquid scintillation counting with [14C] hemoglobin as substrate, and the total alpha‐1‐proteinase inhibitor complex using a sandwich‐immunoassay.Results: The clinical parameters revealed a statistical significant decrease at the 6‐month reexamination. PD levels dropped from 5.40 to 2.88 mm (change 2.52 ± 1.04 mm), the CAL scores from 6.67 to 4.43 mm (change 2.24 ± 0.77 mm). The 30 s GCF volumes dropped from 129.8 to 68.6, displaying a change of 61.1 ± 18.6, p ≤ 0.05. The decrease in total MPO, beta‐NAH and CD levels (medians: 1.7/0.6 µU MPO, 0.035/0.020 µU beta‐NAH, 1.3/0.5 ng CD) following therapy was associated with a significant drop in total GCF amounts of alpha‐1‐EPI from 76.3 ng at baseline to 52.4 ng after 6 months.Conclusion: The clinical healing in chronic periodontal disease is associated with a downregulation of the local PMN responses following periodontal therapy. The reorganization of periodontal tissues is characterized by a decrease of lysosomal enzyme activities and the alpha‐1‐proteinase inhibitor complex in gingival crevicular fluid.