Durable and controlled depletion of neutrophils in mice

Medientyp: E-Artikel

Titel: Durable and controlled depletion of neutrophils in mice

Beteiligte: Boivin, Gael; Faget, Julien; Ancey, Pierre-Benoit; Gkasti, Aspasia; Mussard, Julie; Engblom, Camilla; Pfirschke, Christina; Contat, Caroline; Pascual, Justine; Vazquez, Jessica; Bendriss-Vermare, Nathalie; Caux, Christophe; Vozenin, Marie-Catherine; Pittet, Mikael J.; Gunzer, Matthias; Meylan, Etienne

Erschienen: Springer Science and Business Media LLC, 2020

Sprache: Englisch

DOI: 10.1038/s41467-020-16596-9

ISSN: 2041-1723

Schlagwörter: General Physics and Astronomy ; General Biochemistry, Genetics and Molecular Biology ; General Chemistry ; Multidisciplinary

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Beschreibung: AbstractNeutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies. However, these approaches are only partially effective, transient or lack specificity. Here we report that neutrophils remaining after anti-Ly6G treatment are newly derived from the bone marrow, instead of depletion escapees. Mechanistically, newly generated, circulating neutrophils have lower Ly6G membrane expression, and consequently reduced targets for anti-Ly6G-mediated depletion. To overcome this limitation, we develop a double antibody-based depletion strategy that enhances neutrophil elimination by anti-Ly6G treatment. This approach achieves specific, durable and controlled reduction of neutrophils in vivo, and may be suitable for studying neutrophil function in experimental models.

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