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Ward, Christopher M.; Aumann, Roswitha A.; Whitehead, Mark A.; Nikolouli, Katerina; Leveque, Gary; Gouvi, Georgia; Fung, Elisabeth; Reiling, Sarah J.; Djambazian, Haig; Hughes, Margaret A.; Whiteford, Sam; Caceres-Barrios, Carlos; Nguyen, Thu N. M.; Choo, Amanda; Crisp, Peter; Sim, Sheina B.; Geib, Scott M.; Marec, František; Häcker, Irina; Ragoussis, Jiannis; Darby, Alistair C.; Bourtzis, Kostas; Baxter, Simon W.; Schetelig, Marc F.

White pupae phenotype of tephritids is caused by parallel mutations of a MFS transporter

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  • Medientyp: E-Artikel
  • Titel: White pupae phenotype of tephritids is caused by parallel mutations of a MFS transporter
  • Beteiligte: Ward, Christopher M.; Aumann, Roswitha A.; Whitehead, Mark A.; Nikolouli, Katerina; Leveque, Gary; Gouvi, Georgia; Fung, Elisabeth; Reiling, Sarah J.; Djambazian, Haig; Hughes, Margaret A.; Whiteford, Sam; Caceres-Barrios, Carlos; Nguyen, Thu N. M.; Choo, Amanda; Crisp, Peter; Sim, Sheina B.; Geib, Scott M.; Marec, František; Häcker, Irina; Ragoussis, Jiannis; Darby, Alistair C.; Bourtzis, Kostas; Baxter, Simon W.; Schetelig, Marc F.
  • Erschienen: Springer Science and Business Media LLC, 2021
  • Erschienen in: Nature Communications
  • Sprache: Englisch
  • DOI: 10.1038/s41467-020-20680-5
  • ISSN: 2041-1723
  • Schlagwörter: General Physics and Astronomy ; General Biochemistry, Genetics and Molecular Biology ; General Chemistry ; Multidisciplinary
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  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>Mass releases of sterilized male insects, in the frame of sterile insect technique programs, have helped suppress insect pest populations since the 1950s. In the major horticultural pests <jats:italic>Bactrocera dorsalis, Ceratitis capitata</jats:italic>, and <jats:italic>Zeugodacus cucurbitae</jats:italic>, a key phenotype white pupae (wp) has been used for decades to selectively remove females before releases, yet the gene responsible remained unknown. Here, we use classical and modern genetic approaches to identify and functionally characterize causal <jats:italic>wp</jats:italic><jats:sup>−</jats:sup> mutations in these distantly related fruit fly species. We find that the wp phenotype is produced by parallel mutations in a single, conserved gene. CRISPR/Cas9-mediated knockout of the <jats:italic>wp</jats:italic> gene leads to the rapid generation of white pupae strains in <jats:italic>C. capitata</jats:italic> and <jats:italic>B. tryoni</jats:italic>. The conserved phenotype and independent nature of <jats:italic>wp</jats:italic><jats:sup>−</jats:sup> mutations suggest this technique can provide a generic approach to produce sexing strains in other major medical and agricultural insect pests.</jats:p>
  • Zugangsstatus: Freier Zugang