Erschienen:
Springer Science and Business Media LLC, 2022
Erschienen in:
Nature Communications, 13 (2022) 1
Sprache:
Englisch
DOI:
10.1038/s41467-022-28337-1
ISSN:
2041-1723
Entstehung:
Anmerkungen:
Beschreibung:
AbstractArchaea use a molecular machine, called the archaellum, to swim. The archaellum consists of an ATP-powered intracellular motor that drives the rotation of an extracellular filament composed of multiple copies of proteins named archaellins. In many species, several archaellin homologs are encoded in the same operon; however, previous structural studies indicated that archaellum filaments mainly consist of only one protein species. Here, we use electron cryo-microscopy to elucidate the structure of the archaellum from Methanocaldococcus villosus at 3.08 Å resolution. The filament is composed of two alternating archaellins, suggesting that the architecture and assembly of archaella is more complex than previously thought. Moreover, we identify structural elements that may contribute to the filament’s flexibility.