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Saxena, Vijay; Fitch, James; Ketz, John; White, Peter; Wetzel, Amy; Chanley, Melinda A.; Spencer, John D.; Becknell, Brian; Pierce, Keith R.; Arregui, Sam W.; Nelson, Raoul D.; Schwartz, George J.; Velazquez, Victoria; Walker, Logan A.; Chen, Xi; Yan, Pearlly; Hains, David S.; Schwaderer, Andrew L.

Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function

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  • Medientyp: E-Artikel
  • Titel: Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function
  • Beteiligte: Saxena, Vijay; Fitch, James; Ketz, John; White, Peter; Wetzel, Amy; Chanley, Melinda A.; Spencer, John D.; Becknell, Brian; Pierce, Keith R.; Arregui, Sam W.; Nelson, Raoul D.; Schwartz, George J.; Velazquez, Victoria; Walker, Logan A.; Chen, Xi; Yan, Pearlly; Hains, David S.; Schwaderer, Andrew L.
  • Erschienen: Springer Science and Business Media LLC, 2019
  • Erschienen in: Scientific Reports
  • Sprache: Englisch
  • DOI: 10.1038/s41598-018-36921-z
  • ISSN: 2045-2322
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>The renal collecting duct consists of intercalated cells (ICs) and principal cells (PCs). We have previously demonstrated that collecting ducts have a role in the innate immune defense of the kidney. Transcriptomics is an important tool used to enhance systems-level understanding of cell biology. However, transcriptomics performed on whole kidneys provides limited insight of collecting duct cell gene expression, because these cells comprise a small fraction of total kidney cells. Recently we generated reporter mouse models to enrich collecting duct specific PC and ICs and reported targeted gene expression of anti-microbial peptide genes. Here we report transcriptomics on enriched ICs and PCs and performed a pilot study sequencing four single ICs. We identified 3,645 genes with increased relative expression in ICs compared to non-ICs. In comparison to non-PCs, 2,088 genes had higher relative expression in PCs. IC associated genes included the innate interleukin 1 receptor, type 1 and the antimicrobial peptide (AMP) adrenomedullin. The top predicted canonical pathway for enriched ICs was lipopolysaccharide/Interleukin 1 mediated inhibition of Retinoid X Receptor alpha function and decreased Retinoid X Receptor expression was confirmed to occur 1-hour post experimental murine UTI in ICs but not in non-ICs.</jats:p>
  • Zugangsstatus: Freier Zugang