Erschienen:
Springer Science and Business Media LLC, 2020
Erschienen in:Scientific Reports
Sprache:
Englisch
DOI:
10.1038/s41598-020-77168-x
ISSN:
2045-2322
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>Abstract</jats:title><jats:p><jats:italic>Staphylococcus aureus</jats:italic> is a common cause of catheter-related blood stream infections (CRBSI). The bacterium has the ability to form multilayered biofilms on implanted material, which usually requires the removal of the implanted medical device. A first major step of this biofilm formation is the initial adhesion of the bacterium to the artificial surface. Here, we used single-cell force spectroscopy (SCFS) to study the initial adhesion of <jats:italic>S. aureus</jats:italic> to central venous catheters (CVCs). SCFS performed with <jats:italic>S. aureus</jats:italic> on the surfaces of naïve CVCs produced comparable maximum adhesion forces on three types of CVCs in the low nN range (~ 2–7 nN). These values were drastically reduced, when CVC surfaces were preincubated with human blood plasma or human serum albumin, and similar reductions were observed when <jats:italic>S. aureus</jats:italic> cells were probed with freshly explanted CVCs withdrawn from patients without CRBSI. These findings indicate that the initial adhesion capacity of <jats:italic>S. aureus</jats:italic> to CVC tubing is markedly reduced, once the CVC is inserted into the vein, and that the risk of contamination of the CVC tubing by <jats:italic>S. aureus</jats:italic> during the insertion process might be reduced by a preconditioning of the CVC surface with blood plasma or serum albumin.</jats:p>