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Huber, Charlotte; Stamm, Ivonne; Ziebuhr, Wilma; Marincola, Gabriella; Bischoff, Markus; Strommenger, Birgit; Jaschkowitz, Greta; Marciniak, Tessa; Cuny, Christiane; Witte, Wolfgang; Doellinger, Joerg; Schaudinn, Christoph; Thürmer, Andrea; Epping, Lennard; Semmler, Torsten; Lübke-Becker, Antina; Wieler, Lothar H.; Walther, Birgit

Silence as a way of niche adaptation: mecC-MRSA with variations in the accessory gene regulator (agr) functionality express kaleidoscopic phenotypes

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  • Medientyp: E-Artikel
  • Titel: Silence as a way of niche adaptation: mecC-MRSA with variations in the accessory gene regulator (agr) functionality express kaleidoscopic phenotypes
  • Beteiligte: Huber, Charlotte; Stamm, Ivonne; Ziebuhr, Wilma; Marincola, Gabriella; Bischoff, Markus; Strommenger, Birgit; Jaschkowitz, Greta; Marciniak, Tessa; Cuny, Christiane; Witte, Wolfgang; Doellinger, Joerg; Schaudinn, Christoph; Thürmer, Andrea; Epping, Lennard; Semmler, Torsten; Lübke-Becker, Antina; Wieler, Lothar H.; Walther, Birgit
  • Erschienen: Springer Science and Business Media LLC, 2020
  • Erschienen in: Scientific Reports
  • Sprache: Englisch
  • DOI: 10.1038/s41598-020-71640-4
  • ISSN: 2045-2322
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:p>Functionality of the accessory gene regulator (<jats:italic>agr</jats:italic>) quorum sensing system is an important factor promoting either acute or chronic infections by the notorious opportunistic human and veterinary pathogen <jats:italic>Staphylococcus aureus</jats:italic>. Spontaneous alterations of the <jats:italic>agr</jats:italic> system are known to frequently occur in human healthcare-associated <jats:italic>S. aureus</jats:italic> lineages. However, data on <jats:italic>agr</jats:italic> integrity and function are sparse regarding other major clonal lineages. Here we report on the <jats:italic>agr</jats:italic> system functionality and activity level in <jats:italic>mec</jats:italic>C-carrying methicillin resistant <jats:italic>S. aureus</jats:italic> (MRSA) of various animal origins (n = 33) obtained in Europe as well as in closely related human isolates (n = 12). Whole genome analysis assigned all isolates to four clonal complexes (CC) with distinct <jats:italic>agr</jats:italic> types (CC599 <jats:italic>agr</jats:italic> I, CC49 <jats:italic>agr </jats:italic>II, CC130 <jats:italic>agr</jats:italic> III and CC1943 <jats:italic>agr</jats:italic> IV). Agr functionality was assessed by a combination of phenotypic assays and proteome analysis. In each CC, isolates with varying <jats:italic>agr</jats:italic> activity levels were detected, including the presence of completely non-functional variants. Genomic comparison of the <jats:italic>agr </jats:italic>I–IV encoding regions associated these phenotypic differences with variations in the <jats:italic>agr</jats:italic>A and <jats:italic>agr</jats:italic>C genes. The genomic changes were detected independently in divergent lineages, suggesting that <jats:italic>agr</jats:italic> variation might foster viability and adaptation of emerging MRSA lineages to distinct ecological niches.</jats:p>
  • Zugangsstatus: Freier Zugang