> Detailanzeige

Sendid, Boualem; Lecointe, Karine; Collot, Mayeul; Danzé, Pierre-Marie; Damiens, Sébastien; Drucbert, Anne-Sophie; Fradin, Chantal; Vilcot, Jean-Pierre; Grenouillet, Frédéric; Dubar, Faustine; de Ruyck, Jérôme; Jawhara, Samir; Mallet, Jean-Maurice; Poulain, Daniel

Dissection of the anti-Candida albicans mannan immune response using synthetic oligomannosides reveals unique properties of β-1,2 mannotriose protective epitopes

Literatur-
verwaltung

Zur
Merkliste

Lösche von
Merkliste

Per Whatsapp teilen

Schließen

Merkliste



Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
  • Medientyp: E-Artikel
  • Titel: Dissection of the anti-Candida albicans mannan immune response using synthetic oligomannosides reveals unique properties of β-1,2 mannotriose protective epitopes
  • Beteiligte: Sendid, Boualem; Lecointe, Karine; Collot, Mayeul; Danzé, Pierre-Marie; Damiens, Sébastien; Drucbert, Anne-Sophie; Fradin, Chantal; Vilcot, Jean-Pierre; Grenouillet, Frédéric; Dubar, Faustine; de Ruyck, Jérôme; Jawhara, Samir; Mallet, Jean-Maurice; Poulain, Daniel
  • Erschienen: Springer Science and Business Media LLC, 2021
  • Erschienen in: Scientific Reports
  • Sprache: Englisch
  • DOI: 10.1038/s41598-021-90402-4
  • ISSN: 2045-2322
  • Schlagwörter: Multidisciplinary
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:title>Abstract</jats:title><jats:p><jats:italic>Candida albicans</jats:italic> mannan consists of a large repertoire of oligomannosides with different types of mannose linkages and chain lengths, which act as individual epitopes with more or less overlapping antibody specificities. Although anti-<jats:italic>C. albicans</jats:italic> mannan antibody levels are monitored for diagnostic purposes nothing is known about the qualitative distribution of these antibodies in terms of epitope specificity. We addressed this question using a bank of previously synthesized biotin sulfone tagged oligomannosides (BSTOs) of α and β anomery complemented with a synthetic β-mannotriose described as a protective epitope. The reactivity of these BSTOs was analyzed with IgM isotype monoclonal antibodies (MAbs) of known specificity, polyclonal sera from patients colonized or infected with <jats:italic>C. albicans</jats:italic>, and mannose binding lectin (MBL). Surface plasmon resonance (SPR) and multiple analyte profiling (MAP) were used. Both methods confirmed the usual reactivity of MAbs against either α or β linkages, excepted for MAb B6.1 (protective epitope) reacting with β-Man whereas the corresponding BSTO reacted with anti-α-Man. These results were confirmed in western blots with native <jats:italic>C. albicans</jats:italic> antigens. Using patients’ sera in MAP, a significant correlation was observed between the detection of anti-mannan antibodies recognizing β- and α-Man epitopes and detection of antibodies against β-linked mannotriose suggesting that this epitope also reacts with human polyclonal antibodies of both specificities. By contrast, the reactivity of human sera with other α- and β-linked BSTOs clearly differed according to their colonized or infected status. In these cases, the establishment of an α/β ratio was extremely discriminant. Finally SPR with MBL, an important lectin of innate immunity to <jats:italic>C. albicans</jats:italic>, classically known to interact with α-mannose, also interacted in an unexpected way with the protective epitope. These cumulative data suggest that structure/activity investigations of the finely tuned <jats:italic>C. albicans</jats:italic> anti-mannose immune response are worthwhile to increase our basic knowledge and for translation in medicine.</jats:p>
  • Zugangsstatus: Freier Zugang