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Medientyp:
E-Artikel
Titel:
Evaluation of promoters and ribosome binding sites for biotechnological applications in the unicellular cyanobacterium Synechocystis sp. PCC 6803
Beteiligte:
Englund, Elias;
Liang, Feiyan;
Lindberg, Pia
Erschienen:
Springer Science and Business Media LLC, 2016
Erschienen in:Scientific Reports
Sprache:
Englisch
DOI:
10.1038/srep36640
ISSN:
2045-2322
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>Abstract</jats:title><jats:p>For effective metabolic engineering, a toolbox of genetic components that enables predictable control of gene expression is needed. Here we present a systematic study of promoters and ribosome binding sites in the unicellular cyanobacterium <jats:italic>Synechocystis</jats:italic> sp. PCC 6803. A set of metal ion inducible promoters from <jats:italic>Synechocystis</jats:italic> were compared to commonly used constitutive promoters, by measuring fluorescence of a reporter protein in a standardized setting to allow for accurate comparisons of promoter activity. The most versatile and useful promoter was found to be P<jats:italic>nrsB</jats:italic>, which from a relatively silent expression could be induced almost 40-fold, nearly up to the activity of the strong <jats:italic>psbA2</jats:italic> promoter. By varying the concentrations of the two metal ion inducers Ni<jats:sup>2+</jats:sup> and Co<jats:sup>2+</jats:sup>, expression from the promoter was highly tunable, results that were reproduced with P<jats:italic>nrsB</jats:italic> driving ethanol production. The activities of several ribosomal binding sites were also measured, and tested in parallel in <jats:italic>Synechocystis</jats:italic> and <jats:italic>Escherichia coli</jats:italic>. The results of the study add useful information to the <jats:italic>Synechocystis</jats:italic> genetic toolbox for biotechnological applications.</jats:p>