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Medientyp:
E-Artikel
Titel:
Recruitment of bone marrow CD11b+Gr-1+ cells by polymeric nanoparticles for antigen cross-presentation
Beteiligte:
Yang, Ya-Wun;
Luo, Wen-Hui
Erschienen:
Springer Science and Business Media LLC, 2017
Erschienen in:
Scientific Reports, 7 (2017) 1
Sprache:
Englisch
DOI:
10.1038/srep44691
ISSN:
2045-2322
Entstehung:
Anmerkungen:
Beschreibung:
AbstractThe objective of this study was to investigate the function of poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) on the activation of antigen-specific CD8+T cell responses via the CD11b+Gr−1+myeloid subpopulations in murine bone marrow (BM). PLGA NPs containing ovalbumin (OVA) were fabricated by the double-emulsion method. The CD11b+Gr-1lowLy-6Chighand CD11b+Gr-1highLy-6Clowsubsets from mice bone marrow were sorted and treated with the PLGA/OVA NPs, followed by co-culture with the carboxyfluorescein succinimidyl ester (CFSE)-labelled OT-I CD8+cells. Co-culture of OT-I CD8+T cells with PLGA/OVA NPs-primed CD11b+Gr-1+subsets upregulated the expression of IL-2, TNF-α, INF-γ, granzyme B, and perforin, resulting in proliferation of CD8+T cells and differentiation into effector cytotoxic T lymphocytes (CTLs).In vivoproliferation of CFSE-labelled OT-I CD8+cells in response to OVA was also obtained in the animals immunized with PLGA/OVA NPs. The results presented in this study demonstrate the ability of polymeric NPs to recruit two CD11b+Gr−1+myeloid subsets for effective presentation of exogenous antigen to OT-I CD8+T cells in the context of major histocompatibility complex (MHC) class I, leading to an induction of antigen-specific cell proliferation and differentiation into effector cells.