Kawachi, Shigeyuki;
Jennings, Stephen;
Panes, Julian;
Cockrell, Adam;
Laroux, F. Stephen;
Gray, Laura;
Perry, Michael;
van der Heyde, Henry;
Balish, Edward;
Granger, D. Neil;
Specian, Robert A.;
Grisham, Matthew B.
Cytokine and endothelial cell adhesion molecule expression in interleukin-10-deficient mice
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Medientyp:
E-Artikel
Titel:
Cytokine and endothelial cell adhesion molecule expression in interleukin-10-deficient mice
Beteiligte:
Kawachi, Shigeyuki;
Jennings, Stephen;
Panes, Julian;
Cockrell, Adam;
Laroux, F. Stephen;
Gray, Laura;
Perry, Michael;
van der Heyde, Henry;
Balish, Edward;
Granger, D. Neil;
Specian, Robert A.;
Grisham, Matthew B.
Erschienen:
American Physiological Society, 2000
Erschienen in:American Journal of Physiology-Gastrointestinal and Liver Physiology
Beschreibung:
<jats:p>The objectives of this study were to quantify cytokine mRNA levels and endothelial cell adhesion molecule message and protein expression in healthy wild-type and interleukin-10-deficient (IL-10<jats:sup>−/−</jats:sup>) mice that develop spontaneous and chronic colitis. We found that colonic message levels of IL-1, IL-6, tumor necrosis factor-α, interferon-γ, lymphotoxin-β, and transforming growth factor-β were elevated in colitic mice 10- to 35-fold compared with their healthy wild-type controls. In addition, colonic message levels of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) were found to be increased 10-, 5-, and 23-fold, respectively, in colitic IL-10<jats:sup>−/−</jats:sup>mice compared with their wild-type controls. Immunoradiolabeling as well as immunohistochemistry revealed large and significant increases in vascular surface expression of colonic ICAM-1, VCAM-1, and MAdCAM-1 in the mucosa as well as the submucosa of the colons of colitic mice. These data are consistent with the hypothesis that deletion of IL-10 results in the sustained production of proinflammatory cytokines, leading to the upregulation of adhesion molecules and infiltration of mononuclear and polymorphonuclear leukocytes into the cecal and colonic interstitium.</jats:p>